The frequency of inspection and replacement of parts depends upon the severity of service conditions or the requirements of local, state, and federal rules and regulations. Refer to figure 5 or 6. Reassemble in the reverse order of the above procedures. Before tightening the screw or spring holder key 9 or 20 into the pusher post key 7 to secure the new diaphragm, place the loosely assembled diaphragm and head unit into position in the body, being sure the pusher post is properly hooked on the lever key Rotate the diaphragm so the diaphragm and body holes align.
Tighten the screw or reset stem and proceed with reassembly. Turn the adjusting screw to about mid position. This will stretch the oversized diaphragm to ensure slack in the assembled diaphragm. The slack created by this method is necessary for good regulation. Be sure the diaphragm does not fold over at the flange with reassembling. Replace the valve disk or seat ring if either is nicked, cut, or otherwise damaged. Reassemble in the reverse order of the above procedure. Treat the male threads of the new seat ring with pipe compound before reassembling.
Always provide this information in any correspondence with your Fisher representative regarding replacement parts or technical assistance. If construction changes are made in the field, be sure that the closing cap is also changed to reflect the most recent construction. If, within one year from the date the product is shipped from the factory, any part fails because of a manufacturing defect, FISHER will provide a free replacement part for any defective or malfunctioning part, FOB McKinney, Texas.
This warranty applies only when the product is used for consumer use within the United States and is installed and used in accordance with all applicable national, state and local codes, regulations and laws. This warranty shall not apply if the product has been subjected to unreasonable use, negligence, accident in transit, alteration, improper.
While this information is presented in good faith and believed to be accurate, Fisher Controls does not guarantee satisfactory results from reliance upon such information.
Nothing contained herein is to be construed as a warranty or guarantee, express or implied, regarding the performance, merchantability, fitness. FISHER shall not be liable for any default or delay in performance under this warranty caused by any contingency beyond its control, including without limitation, war, government restrictions or restraint, strikes, fire, flood, or a short or reduced supply of raw material.
Fisher Controls reserves the right, without notice, to alter or improve the designs or specifications of the products described herein. Millions discover their favorite reads on issuu every month. Give your content the digital home it deserves.
Get it to any device in seconds. There must always be a sample loop or Superloop connected to ports 2 and 6 of the injection valve. This is to prevent liquid spraying out of the ports when switching the valve. If the system is configured for sample application directly onto the column using an optional sample pump, a tubing must be connected between ports 3 and 6.
Optional configurations are selected, installed and implemented by the user. An optional configuration consists of both hardware components and software instructions. Extra rack panel for optional equipment. The chapter also discusses how methods are selected and system handling while preparing a run section 2. A comprehensive list of the recommended pre-packed columns is given overleaf.
On delivery, the system is equipped with i. When running columns with a low maximum pressure and high flow rates, PEEK tubing with a larger inner diameter may be used instead to prevent increased back-pressure after columns, which could cause the columns to rupture.
Note: It is very important to use the correct tubing diameter and to take into consideration the maximum allowed pressure for the column and the size of the column.
This is achieved by the following method: 1. Clicking a column in the list will display its parameters in the field to the right of the box. Click Edit to display the Edit Column dialog. In the Parameter column, enter in the field for Max pressure the new unit pressure limit, 0. Click Replace after the new value has been entered. Click Save as and enter a new name of your column. You can choose to save the column globally, i.
However, we recommend to clear the Save as global box in this situation. Click OK. Click Save as again to add the updated column parameters to the column list. Note: The inlet port of the 5 mm UV cell is above the optical unit. The inlet port of the 2 mm UV cell is below the optical unit.
For application of large sample volumes, Superloops and a sample pump are available as options. The following table shows which technique is recommended for different sample volumes. Sample application technique. Refer also to the User Manual for the sample pump. Manual sample injection is selected in the Sample Injection page in the Method Wizard.
The seal should allow easy insertion and removal of the needle. Note: If the syringe is taken out before the sample is injected onto the column, self-drainage can occur and the loop will be emptied. If used, the Union Luer connector replaces the injection fill port in port 3 of the injection valve. Two techniques can be used for filling the sample loop; partial or complete filling.
Type of filling. The volumetric accuracy and precision is that of the syringe. Partial filling allows the injected volume to be changed without changing the loop and does not waste sample.
The sample loop must be completely filled with buffer before the sample can be loaded. Partial filling is achieved as follows: 1. Insert the syringe into the injection fill port on the injection valve.
Set the injection valve to position LOAD. Leave the syringe in position. For analytical reproducibility, a sample volume 5 times the volume of the sample loop should be used. Five loop volumes ensure better precision. With complete filling, the sample volume can only be changed by changing the loop size.
Complete filling is achieved as follows: 1. Note: If the syringe is taken out before the sample is injected onto the column, self-drainage will occur and the loop will be emptied. The volume for emptying the sample loop is set in the Sample Injection dialog in the Method Wizard.
The output flow from the pump is routed to Mixer M Other mixer chambers with 2 ml and 5 ml mixer volumes are available as accessories. See section 5. When using eluents that are more difficult to mix, such as isopropanol and water, a larger mixer volume will give better mixing. Note: If the pH optional and conductvity curves indicate uneven mixing of your buffers unstable curves , change to a 2 ml or 5 ml mixer chamber.
The flow cell can be changed when required, for example from 2 mm to 5 mm to increase the sensitivity, or from 5 mm to 2 mm to decrease the sensitivity. Change the flow cell as follows: 1.
The inlet port of the 5 mm UV cell is above the optical unit. If the locking nut is not tightened sufficiently, the monitor will function poorly e.
Place the protective cover around the flow cell to protect the electronics inside the optical unit from liquid spillage. The Hg lamp housing has two positions, one for nm, marked by on the filter housing, and the other one marked by for all other wavelengths. The Zn lamp housing has only one position, for nm. There will be a faint click when the housing is correctly slide into either position.
Set the wavelength to be used by selecting lamp position indicated by a dot on the lamp housing in combination with the appropriate filter, i. A click will indicate that the filter is in position. This must be entered by the user. When starting a method, a question appears in which the user is prompted to state the wavelength to be used in the run. Fractionation is selected and specified in the fractionation dialogs in the Method Wizard. This fractionation method is available in all methods except gel filtration methods.
Fixed volume fractionation can be combined with peak fractionation, which means collecting peaks during elution. The peak size is set to a volume which is larger than the actual peak volumes.
The peak size is set to a volume which is smaller than the actual peak volume. The basic steps required to create a method are: 1. Select the appropriate parameter values in the subsequent pages. Click OK to go to the next page. Check and fine-tune the values for the method variables, e. Before starting the run, check that the system is in End mode, and that the valves are in their initial positions.
Conductivity flow cell Cell constant Temperature Entering a new cell constant Sample pump optional. Must be done when changing the conductivity flow cell Temp. Whenever the running conditions are changed.
Check that the waste bottle is not full and will accept the volume diverted to it during the run. Check that the pump has been purged i. If not, purge the pump as described in the P User Manual. Check that the correct wavelength is set on the optical unit and that the correct UV flow cell is installed. Click the Customize panes toolbar button or choose View:Panes from the menu to select which panes to display.
Run Data. The run data pane displays the current values for selected run parameters. Right-click in the Run Data pane and select Properties. Select the run data items to be displayed and click OK.
The curves pane displays the monitor signal values graphically. Rightclick in the Curves pane and select Properties to select the curves to display. All curves are always stored in the result file. Flow scheme. The flow scheme is a graphical representation of the flow path in the chromatography system. During a run, the flow scheme shows open flow paths and monitor signals with numerical displays.
All actions and unexpected conditions such as warnings are logged for every run, with date, time and current username. The logbook provides a complete history of any given run. The log is saved in the result file. Ratings and reviews Write a review.
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